In teaching about semen analysis, which statement should be included?

• A. Refrigerate the specimen after collection
• B. Abstain from ejaculation for at least 2 to 5 days prior to the test
• C. Leave the specimen at room temperature for 3 to 4 hr prior to transport to the laboratory
• D. Collect the specimen using a condom with spermicidal lubricant

Answer: (B)

Standard semen analysis relies on a controlled abstinence period to produce consistent, interpretable results. When a person waits about two to five days after ejaculation before providing a sample, the semen volume, sperm concentration, and motility are more stable and comparable across tests and individuals. If abstinence is shorter, the sample may have lower volume and potentially misleading concentration; if abstinence is longer, changes in volume and sperm characteristics can also skew results. This 2–5 day window is the practical guideline to reduce variability and improve the reliability of the analysis.

Refrigerating the specimen can alter sperm function and isn’t the standard handling practice. Leaving the sample at room temperature for several hours before testing can degrade motility and viability, leading to inaccurate results. Collecting with a condom that contains spermicidal lubricant introduces chemicals that impair sperm, making the sample unsuitable for analysis.